2 edition of role of individual domains of eIF4G in translation initiation found in the catalog.
role of individual domains of eIF4G in translation initiation
Written in English
D.Phil. 2000. BLDSC DXN036535.
|Statement||[by] Wendy Wood.|
|Series||Sussex theses ; S 4924|
Translation is a fundamental step in gene expression that regulates multiple developmental and stress responses. One key step of translation initiation is the association between eIF4E and : René Toribio, Alfonso Muñoz, Alfonso Muñoz, Ana B. Castro-Sanz, Catharina Merchante, M. Mar Castella. View protein in InterPro IPR ARM-type_fold IPR Initiation_fac_eIF4g_MI IPR MIF4-like_sf IPR MIF4G-like_typ-3 IPR W2_domain Pfam i View protein in Pfam PF MA3, 1 hit PF MIF4G, 1 hit PF W2, 1 hit.
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by the eIF4G2 gene shares similarity with the C-terminal region Aliases: EIF4G2, AAG1, DAP5, NAT1, . Core Components of the Translation Initiation Complex Are Modified by O-GlcNAc. To investigate whether OGT physically associates with the translation initiation complex eIF4F in cells, we performed pull-down assays using m 7 GDP affinity beads. Consistent with previous studies, eIF4F was efficiently isolated from T cell lysates (Fig. 1A) ().OGT Cited by: 3.
Communication between the 5′ cap structure and 3′ poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor eIF4G. Here, we demonstrate the reconstitution of an eIF4E/eIF4G Cited by: factors connected to the 40S [12,13,14]. In addition to its role in canonical translation initiation, eIF4G is also required for 59 cap-independent translation of some host and viral mRNAs [15,16,17]. Interestingly, the genomes of diverse eukaryotes encode more than one form of eIF4G. Previous work suggested that eIF4G.
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Role in aging. Regulation of translation initiation by eIF4G is vital for protein synthesis in developing organisms, for example yeast and nematodes. Deletion of eIF4G is lethal in yeast. In the roundworm C. elegans, knockout of eIF4G leads to animals that cannot develop past the early larval stage (L2) of development.
Inhibiting expression of eukaryotic translation initiation factor 4G (eIF4G) arrests normal development but extends lifespan when suppressed during adulthood. In addition to reducing overall translation, inhibition alters the stoichiometry of mRNA translation in favor of genes important for responding to stress and against those associated with growth and reproduction in C.
by: The role of individual domains of eIF4G in translation initiation. (Thesis) Wood W. Publisher: University of Sussex  Metadata Source: The British Library Type: Thesis. Abstract. No abstract supplied. Menu. Formats. Abstract.
EThOS. About. About Europe PMC; Funders; Joining Europe PMC Author: Wood W. The role of individual domains of eIF4G in translation initiation. Author: Wood, Wendy. ISNI: Awarding Body: University of Sussex Current Institution: University of Sussex Date of Award: Availability of Full Text.
Here, we briefly review the general role of eIF4G in translation initiation and then discuss what is known about the functional importance of the timing, tissue-specificity, and form (homolog, splice variant, or cleavage product) of this factor, especially with regard to lifespan determination and age-related by: Diagram of eIF4G shows the placement and relative sizes of the domains known to bind other proteins involved in translation: polyA binding protein (PABP) (Aplysia.
The eukaryotic translation initiation factor, eIF4G, plays a key functional role in the initiation of cap-dependent translation by acting as an adapter to nucleate the assembly of eIF4F complex.
eIF4A is a DEAD-box helicase involved in translation initiation. (A) Scheme of translation initiation in eukaryotes. The DEAD-box helicase eIF4A (yellow), the cap-binding protein eIF4E (red) and the scaffold protein eIF4G (green) form the eIF4F complex Cited by: Translation driven by an eIF4G core domain in vivo Article (PDF Available) in The EMBO Journal 18(17) October with 67 Reads How we measure 'reads'.
Eukaryotic translation initiation factor 4G (eIF4G) plays a critical role in protein expression, and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5‘-end of mRNA and promotes the assembly of a functional translation initiation complex, which scans along the mRNA to the translation Cited by: A 43S complex comprising a 40S ribosomal subunit in association with initiator tRNA and eukaryotic initiation factors (eIFs) binds to the capped 5′ end of an mRNA (step 1) and scans downstream along the 5′-untranslated region (5′-UTR; step 2) until it encounters the first AUG triplet and stops (step 3).Cited by: Initiation of translation also involves the interaction of key factors with the 5’-end of an mRNA molecule, the 5’-cap, as well as with the 5’-UTR.
The eIF4F complex mediates cap-dependent translation initiation, a multisubunit complex composed of several proteins, including eIF4A, eIF4B, eIF4E, and eIF4G. Gonatopoulos-Pournatzis et al. demonstrate that conserved, autism-disrupted microexons in eIF4G translation initiation factors regulate the neuronal proteome and control higher order cognitive functions.
The microexons function as a translational brake and elicit ribosome stalling on transcripts encoding synaptic proteins through their propensity to coalesce with FMRP and Cited by: 1. Protein translation is orchestrated by an assembly of various protein components at the ribosomal subunits. The eukaryotic translation initiation factor 4G (eIF4G) plays an important role in the formation of the translation initiation complex eIF4F consisting of eIF4G, the ATP dependent RNA helicase eIF4A and the cap binding protein by: 4.
eIF4GI possesses three domains that are roughly equivalent in size: the N-terminal part as defined by its cleavage by picornaviral proteases, the middle ‘core’ domain that is critical for assembly of the translation machinery and the carboxy-terminal fragment which appears to play a modular role in by: eIF4G is a large factor which is thought to play a scaffolding role, coordinating interactions between translation initiation factors such that, in the steady state, eIF4G exists in the eIF4F complex with by: First, we evaluated whether the role of eIF4G–Pab1–eRF3 interactions in the formation of a closed loop (and, possibly, ribosome recycling 24) was also dependent on translation termination 24 Cited by: Eukaryotic translation initiation factor 4G (eIF4G) functions to organize the assembly of initiation factors required for the recruitment of a 40S ribosomal subunit to an mRNA and for interacting with the poly(A) binding protein (PABP).
Many eukaryotes express two highly similar eIF4G isoforms. eIFiso4G, one of two isoforms in plants, is highly divergent and unusually Cited by: Translation initiation requires the formation of a pre-initiation complex that recruits the 5′ end of the mRNA and scans along it to locate the start codon.
Genetic, biochemical and structural Cited by: Eukaryotic initiation factor 4F (eIF4F) plays a critical role in this process and consists of three subunits: eIF4E, a protein that binds mRNA cap structures (m 7 GpppN, where N is any nucleotide) present at the 5′ end of cellular cytoplasmic mRNAs; eIF4A, an ATP-dependent RNA helicase; and eIF4G, a scaffolding protein that recruits 43S Cited by:.
The 40S-eIF3-IRES model allows us to infer the placement of eIF4G relative to 40S and other initiation factors, locating eIF4G very close to the E site (movie S1). This analysis predicts the position of eIF4F and the approximate location of the 5′-m7G cap of an by: Eukaryotic translation initiation factor 4G (eIF4G) functions to organize the assembly of initiation factors required for the recruitment of a 40S .Eukaryotic initiation factors (eIFs) are proteins or protein complexes involved in the initiation phase of eukaryotic proteins help stabilize the formation of ribosomal preinitiation complexes around the start codon and are an important input for post-transcription gene l initiation factors form a complex with the small 40S ribosomal .